{"id":2500,"date":"2022-10-30T15:17:00","date_gmt":"2022-10-30T15:17:00","guid":{"rendered":"http:\/\/www.gongyeqx.com\/?p=2500"},"modified":"2022-09-05T15:17:26","modified_gmt":"2022-09-05T07:17:26","slug":"6-fam-mal%ef%bc%8c6-%e7%be%a7%e5%9f%ba%e8%8d%a7%e5%85%89%e7%b4%a0-%e9%a9%ac%e6%9d%a5%e9%85%b0%e4%ba%9a%e8%83%ba-%e7%9a%84%e5%8c%96%e5%ad%a6%e6%80%a7%e8%b4%a8","status":"publish","type":"post","link":"http:\/\/www.gongyeqx.com\/?p=2500","title":{"rendered":"6-FAM-Mal\uff0c6-\u7fa7\u57fa\u8367\u5149\u7d20-\u9a6c\u6765\u9170\u4e9a\u80fa \u7684\u5316\u5b66\u6027\u8d28"},"content":{"rendered":"

\u82f1\u6587\u540d\uff1a<\/strong>FAM maleimide, 6-isomer<\/span><\/p>\n

\u5206\u5b50\u5f0f\uff1a<\/strong>C27H18N2O8<\/span><\/p>\n

\u5206\u5b50\u91cf\uff1a<\/strong>498.44<\/span><\/p>\n

\u7ed3\u6784\u5f0f\uff1a<\/strong><\/span><\/p>\n

<\/p>\n

\u7eaf\u5ea6\uff1a<\/strong>95%<\/span><\/p>\n

\u6eb6\u89e3\u5ea6\uff1a<\/strong>DMSO\u548cDMF\u90fd\u5f88\u597d<\/span><\/p>\n

\u50a8\u5b58\u6761\u4ef6\uff1a<\/strong>\u5728-20\u00b0C\u7684\u9ed1\u6697\u4e2d24\u4e2a\u6708<\/span><\/p>\n

\u8fd0\u8f93\uff1a<\/strong>\u5728\u5ba4\u6e29\u4e0b\u6700\u591a3\u5468\u3002\u907f\u514d\u957f\u65f6\u95f4\u66b4\u9732\u5728\u9633\u5149\u4e0b<\/span><\/p>\n

\u8fd0\u8f93\u6761\u4ef6\uff1a<\/strong>\u4f4e\u6e29\u8fd0\u8f93<\/span><\/p>\n

\u5316\u5b66\u6027\u8d28\uff1a<\/strong>FAM\uff08\u8367\u5149\u7d20\uff09\u662f\u4e00\u79cd\u4e0e\u5404\u79cd\u8367\u5149\u68c0\u6d4b\u4eea\u5668\u517c\u5bb9\u7684\u660e\u4eae\u8367\u5149\u56e2\u3002FAM\u662f\u4e00\u79cd\u901a\u7528\u67d3\u6599\uff0c\u53ef\u7528\u4e8e\u663e\u5fae\u955c\u3001qPCR\u548c\u8bb8\u591a\u5176\u4ed6\u65b9\u6cd5\uff0c\u4ee5\u53ca\u57fa\u4e8eFRET\u548c\u8367\u5149\u504f\u632f\u7684\u7ed3\u5408\u5206\u6790\u3002<\/span><\/p>\n

FAM\u9a6c\u6765\u9170\u4e9a\u80fa\u662f\u4e00\u79cd\u5def\u57fa\u6d3b\u6027\u67d3\u6599\uff0c\u7528\u4e8e\u6807\u8bb0\u86cb\u767d\u8d28\u3001\u80bd\u548c\u5176\u4ed6\u786b\u5316\u5206\u5b50\u3002<\/span><\/p>\n

<\/span><\/p>\n

6-FAM\u662f\u7fa7\u57fa\u8367\u5149\u7d20\u7684\u4e00\u4e2a\u540c\u5206\u5f02\u6784\u4f53\uff0c\u4e3b\u8981\u7528\u4e8e\u6838\u9178\u7684\u6d4b\u5e8f\u548c\u6807\u8bb0\u3002<\/span><\/p>\n

6-FAM\uff086-\u7fa7\u57fa\u8367\u5149\u7d20\uff09\u542b\u6709\u4e00\u79cd\u7fa7\u9178\uff0c\u53ef\u901a\u8fc7\u7fa7\u9178\u7684\u78b3\u4e8c\u4e9a\u80fa\u6d3b\u5316\u4e0e\u4f2f\u80fa\u53cd\u5e94\u3002\u8367\u5149\u7d20\u662f\u6807\u8bb0\u751f\u7269\u5206\u5b50\u5e38\u7528\u7684\u8367\u5149\u884d\u751f\u8bd5\u5242\u3002\u9664\u4e86\u5177\u6709\u76f8\u5bf9\u8f83\u9ad8\u7684\u5438\u6536\u7387\u3001\u5f88\u597d\u7684\u8367\u5149\u91cf\u5b50\u4ea7\u7387\u548c\u826f\u597d\u7684\u6c34\u6eb6\u6027\u5916\uff0c\u8367\u5149\u7d20\u8fd8\u5177\u6709\u4e0e\u6c29\u79bb\u5b50\u6fc0\u5149488 nm\u8c31\u7ebf\u5bc6\u5207\u5339\u914d\u7684\u6fc0\u53d1\u3002 <\/span><\/p>\n

<\/p>\n

<\/span><\/p>\n

\u4f9b\u5e94\u5546\uff1a<\/strong>\u897f\u5b89\u51ef\u65b0\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8<\/strong>
<\/span><\/p>\n

\u8bf7\u70b9\u51fb\u6b64\u5904<\/strong>\u897f\u5b89\u51ef\u65b0\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u8fdb\u5165\u5c55\u53f0\u67e5\u770b<\/strong><\/span><\/p>\n

\u8bf7\u70b9\u51fb\u6b64\u5904<\/strong>\u897f\u5b89\u51ef\u65b0\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8<\/strong><\/span>\u8fdb\u5165\u5c55\u53f0\u67e5\u770b<\/strong><\/span><\/p>\n

\u897f\u5b89\u51ef\u65b0\u751f\u7269\u79d1\u6280\u6709\u9650\u516c\u53f8\u662f\u56fd\u5185\u7684\u70b9\u51fb\u5316\u5b66\u8bd5\u5242\u4f9b\u5e94\u5546\uff0c\u4ea7\u54c1\u5e94\u7528\u4e8e\u5404\u5927\u79d1\u7814\u9662\u6821\u3001\u68c0\u9a8c\u673a\u6784\u3001\u73af\u5883\u76d1\u6d4b\u3001\u751f\u7269\u5236\u836f\u3001\u77f3\u6cb9\u5316\u5de5\u3001\u822a\u5929\u6280\u672f\u3001\u516c\u5171\u5b89\u5168\u7b49\u4f17\u591a\u9886\u57df\u3002
\u6211\u516c\u53f8\u63d0\u4f9b\u7684\u70b9\u51fb\u7c7b\u5316\u5b66\u8bd5\u5242\u5305\u62ec\uff1aDBCO\u3001TCO\u3001Tetrazine\u3001Azide\u3001Alkyne\u3001Auxiliary reagents\u7b49\u3002\u5176\u4e2dDBCO Reagents\u53c8\u5305\u542b\u4e86\u4e0e\u6c28\u57fa\u53cd\u5e94\u7684\uff0c\u4e0e\u7fa7\u57fa\u53cd\u5e94\u7684\uff0c\u4e0e\u751f\u7269\u7d20\u4ea4\u8054\u7684\uff0c\u4e0e\u8367\u5149\u7d20\u4ea4\u8054\u7684\uff0c\u4e0ePEG\u4ea4\u8054\u7684\u7b49\u7b49\u4ea7\u54c1\u3002\u5404\u8bd5\u5242\u5177\u6709\u591a\u6837\u7684\u89c4\u683c\u7684\u5305\u88c5\uff0c\u80fd\u6ee1\u8db3\u60a8\u5404\u7c7b\u5b9e\u9a8c\u9700\u6c42\uff0c\u897f\u5b89\u51ef\u65b0\u6240\u6709\u5458\u5de5\u5e0c\u671b\u80fd\u4e0e\u60a8\u643a\u624b\u5171\u8fdb\uff0c\u5171\u521b\u8f89\u714c\u3002<\/span><\/p>\n


<\/span><\/p>\n

<\/p>\n

\u5176\u4ed6\u4ea7\u54c1\u5217\u8868\uff1a<\/strong><\/span><\/p>\n

FAM DBCO, 6-isomer <\/span><\/span><\/p>\n

FAM NHS ester, 6-isomer <\/span><\/span><\/p>\n

FAM azide, 6-isomer<\/span><\/p>\n

FAM-11-dCTP, 6-isomer <\/span><\/span><\/p>\n

FAM CPG 500, 6-isomer<\/span><\/p>\n

5-Carboxy Fluorescein\uff0c5-FAM<\/span><\/p>\n

6-Carboxy Fluorescein\uff0c6-FAM<\/span><\/p>\n

5(6)Carboxy fluorescein\uff0c5(6)-FAM<\/span><\/p>\n

5(6)-FAM, SE<\/span><\/p>\n

5-Carboxyfluorescein succinimidyl ester\uff0c5-FAM, SE<\/span><\/p>\n

6-Carboxyfluorescein succinimidyl ester\uff0c6-FAM, SE<\/span><\/p>\n

<\/p>\n<\/p><\/div>\n<\/p><\/div>\n","protected":false},"excerpt":{"rendered":"

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